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This study was performed to find HBsAg and Anti-HBs prevalence between the two groups. One group, defined as occupationally risk group, consists of persons who treat patients personally or who deal with blood as their daily activity. The other group, defined as occupationally non-risk group, consists of remaining persons in the study. The data were collected from the annual screening physical check-up which was performed in 1984 for 2,631 teaching, clerical and other personnel of Y university. RPHA method was used for the detection of HBsAg and Anti-HBs. The results from the analysis are summarized as follows: 1) No difference was observed in HBsAg positive rate between occupationally risk (6.9%) and non-risk group(7.1%) but showed significant difference in HBsAg or Anti-HBs positive rate between the two groups(24.2% and 19.4%, P<0.01). This finding suggests that there is higher risk to expose to Hapatitis B Virus(HBV) in occupationally risk group than in non-risk one. 2) Difference in HBsAg or Anti-HBs positive rate between occupationally risk and non-risk group was statistically significant for the 20~29 age group but not for the remaining age groups. This finding suggests that vaccination against hepatitis B(HB) is highly recomrmanded to this age group especially for those who take this highly risk occupation.