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Three hundred sera were titered to examine the presence of complement-fixing (CF) antibodies to the cytomegalovirus (CMV), which were randomly sampled among blood units of 10,241 volunteer donors in Seoul area from February to March 1981. The objective of the study was to identify the frequency and distribution of CMV CF antibody among blood donors in Seoul, of which information has not yet been reported in Korea. The results obtained are as follows ; 1) Two hundred forty-two of 300 donors, aged 16 to 40 years, yielded positive CF test by micro titration technique at titers ranging from 1:4 to 1:32 (positive rate 81%). 2) The positive rate of CMV CF antibody by sex and occupation did not show any statistically significant differences. 3) The percent of CMV CF antibody positive was 87% (104 out of 120) among child-bearing women (16 through 40 of age). Although the results show high incidence of C-F antibodies to CMV, it is difficult to detect the agent for CMV infection efficiently from the blood of infected donors under the current status of knowledge and techniques. Further studies are called for to provide the information necessary for a wise decision to exclude the blood of CMV infeeted donors from use in transfusion.