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Copro-molecular diagnosis of the Toxoplasmatinae subfamily in dog and cat populations in northern Iran
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Leila Izadi, Shahabeddin Sarvi, Seyed Abdollah Hosseini, Afsaneh Amouei, Mehdi Sharif, Mohammad Taghi Rahimi, Tooran Nayeri, Ahmad Daryani
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Epidemiol Health. 2020;42:e2020074. Published online December 4, 2020
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DOI: https://doi.org/10.4178/epih.e2020074
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Abstract
OBJECTIVES
The oocysts of the Toxoplasmatinae subfamily (<i>Neospora caninum, Hammondia hammondi</i> and <i>H. heydorni</i>, and <i>Besnoitia besnoiti</i>) are morphologically similar to <i>Toxoplasma gondii</i>, and indistinguishable from each other. This study investigated the prevalence of the Toxoplasmatinae subfamily in dog and cat fecal samples using a nested polymerase chain reaction method.
METHODS Overall, 200 fecal samples from domestic dogs (n=120) and cats (n=80) were collected from 15 farms in northern Iran. The samples were homogenized in 2.5% potassium dichromate solution and subsequently concentrated with sucrose solution. DNA was extracted from samples using a genomic DNA kit. Specific primers and the 18S rDNA gene were used to screen and detect all Toxoplasmatinae oocysts.
RESULTS Overall, 2.5% (3 of 120) and 22.5% (18 of 80) of the fecal samples collected from dogs and cats were infected with Toxoplasmatinae. In dogs, 2 samples were positive for <i>N. caninum</i> and 1 sample was positive for <i>T. gondii</i>. In cats, all 18 positive samples belonged to <i>T. gondii</i>. No contamination with <i>H. heydorni</i> was observed in dog fecal samples or <i>H. hammondi</i> and <i>B. besnoiti</i> in cat fecal samples. A phylogenetic analysis revealed that the <i>T. gondii</i> (cat) and <i>N. caninum</i> (dog) found had similarities with parasites reported from other regions of the world.
CONCLUSIONS This is the first study to provide data on the epidemiology of Toxoplasmatinae oocysts in Iran. The findings suggest that public-health monitoring for the effective control of feces from cats and dogs and improved pet hygiene habits are needed.
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Summary
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Citations
Citations to this article as recorded by  - Prevalence of Toxoplasma gondii in Endangered Wild Felines (Felis silvestris and Lynx pardinus) in Spain
Pablo Matas Méndez, Isabel Fuentes Corripio, Ana Montoya Matute, Begoña Bailo Barroso, Rebeca Grande Gómez, Ariadna Apruzzese Rubio, Francisco Ponce Gordo, Marta Mateo Barrientos
Animals.2023; 13(15): 2488. CrossRef - Endoparasites of European Wildcats (Felis silvestris) in Greece
Anastasia Diakou, Despina Migli, Dimitris Dimzas, Simone Morelli, Angela Di Cesare, Dionisios Youlatos, Petros Lymberakis, Donato Traversa
Pathogens.2021; 10(5): 594. CrossRef
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